Anti-stokes fluorescence of phycobilisome and its complex with the orange carotenoid protein

Zlenko, D.V.; Protasova, E.A.; Tsoraev, G.V.; Sluchanko, N.N.; Cherepanov, D.A.; Friedrich, T.; Ge, B.; Qin, S.; Maksimov, E.G.; Rubin, A.B.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 15 февраля 2024 г.

Авторы: Zlenko Dmitry V., Protasova Elena A., Tsoraev Georgy V., Sluchanko Nikolai N., Cherepanov Dmitry A., Friedrich Thomas, Ge Baosheng, Qin Song, Maksimov Eugene G., Rubin Andrew B.
Журнал: Biochimica et Biophysica Acta - Bioenergetics
Том: 1865
Номер: 1
Год издания: 2024
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Номер статьи: 149014
DOI: 10.1016/j.bbabio.2023.149014
Аннотация: Phycobilisomes (PBSs) are giant water-soluble light-harvesting complexes of cyanobacteria and red algae, consisting of hundreds of phycobiliproteins precisely organized to deliver the energy of absorbed light to chlorophyll chromophores of the photosynthetic electron-transport chain. Quenching the excess of excitation energy is necessary for the photoprotection of photosynthetic apparatus. In cyanobacteria, quenching of PBS excitation is provided by the Orange Carotenoid Protein (OCP), which is activated under high light conditions. In this work, we describe parameters of anti-Stokes fluorescence of cyanobacterial PBSs in quenched and unquenched states. We compare the fluorescence readout from entire phycobilisomes and their fragments. The obtained results revealed the heterogeneity of conformations of chromophores in isolated phycobiliproteins, while such heterogeneity was not observed in the entire PBS. Under excitation by low-energy quanta, we did not detect a significant uphill energy transfer from the core to the peripheral rods of PBS, while the one from the terminal emitters to the bulk allophycocyanin chromophores is highly probable. We show that this direction of energy migration does not eliminate fluorescence quenching in the complex with OCP. Thus, long-wave excitation provides new insights into the pathways of energy conversion in the phycobilisome.
Добавил в систему: Максимов Евгений Георгиевич

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Anti-stokes fluorescence of phycobilisome and its complex with the orange carotenoid proteinстатья

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