Incorporation of Pectin into Vaterite Microparticles Prevented Effects of Adsorbed Mucin on Neutrophil Activation

Mikhalchik, E.V.; Maltseva, L.N.; Firova, R.K.; Murina, М.А.; Gorudko, I.V.; Grigorieva, D.V.; Ivanov, V.A.; Obraztsova, E.A.; Klinov, D.V.; Shmeleva, E.V.; Gusev, S.A.; Panasenko, O.M.; Sokolov, A.V.; Gorbunov, N.P.; Filatova, L.Y.; Balabushevich, N.G.

Авторы: Mikhalchik E.V., Maltseva L.N., Firova R.K., Murina М.А., Gorudko I.V., Grigorieva D.V., Ivanov V.A., Obraztsova E.A., Klinov D.V., Shmeleva E.V., Gusev S.A., Panasenko O.M., Sokolov A.V., Gorbunov N.P., Filatova L.Y., Balabushevich N.G.
Журнал: International Journal of Molecular Sciences
Том: 24
Год издания: 2023
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Номер статьи: 15927
DOI: 10.3390/ijms242115927
Аннотация: The application of vaterite microparticles for mucosal delivery depends on their interactionwith mucin and immune cells. As we have shown previously, the binding of mucin onto particlesenhances the generation of reactive oxygen species by neutrophils. The attenuation of the pro-oxidanteffect of the bound mucin through the modification of vaterite could improve its biocompatibility.Hybrid microparticles composed of vaterite and pectin (CCP) were prepared using co-precipitation.In comparison with vaterite (CC), they had a smaller diameter and pores, a greater surface area,and a negative zeta-potential. We aimed to study the cytotoxicity and mucin-dependent neutrophilactivating effect of CCP microparticles. The incorporated pectin did not influence the neutrophildamage according to a lactate dehydrogenase test. The difference in the CC- and CCP-elicited luminolor lucigenin chemiluminescence of neutrophils was insignificant, with no direct pro- or antioxidanteffects from the incorporated pectin. Unlike soluble pectin, the CCP particles were ineffective atscavenging radicals in an ABAP–luminol test. The fluorescence of SYTOX Green demonstrated aCCP-stimulated formation of neutrophil extracellular traps (NETs). The pre-treatment of CC andCCP with mucin resulted in a 2.5-times-higher CL response of neutrophils to the CC-mucin than tothe CCP-mucin. Thus, the incorporation of pectin into vaterite microspheres enabled an antioxidanteffect to be reached when the neutrophils were activated by mucin-treated microparticles, presumablyvia exposed ligands.
Добавил в систему: Балабушевич Надежда Георгиевна

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Incorporation of Pectin into Vaterite Microparticles Prevented Effects of Adsorbed Mucin on Neutrophil Activationстатья

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