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Rol' giperékspressii DNK-metiltransferaz v persistentsii kol'tsevoĭ kovalentno zamknutoĭ DNK pri khronicheskom gepatite V [Overexpression of DNA-methyltransferases in persistency of cccDNA pool in chronic hepatitisстатья
Статья опубликована в журнале из списка RSCI Web of Science
Статья опубликована в журнале из перечня ВАКСтатья опубликована в журнале из списка Web of Science и/или Scopus
- Авторы: Kostyushev D.S., Zueva A.P., Brezgin S.A., Lipatnikov A.D., Simirskii V.N., Glebe D., Volchkova E.V., Shipulin G.A., Chulanov V.P.
- Журнал: Терапевтический архив
- Том: 89
- Номер: 11
- Год издания: 2017
- Издательство: Медиа Сфера
- Местоположение издательства: М.
- Первая страница: 21
- Последняя страница: 26
- Аннотация: Aim: To define the role of DNA-methyltransferases of type 1 and type 3A in hepatitis B viral cycle. Material and methods: Human hepatoma cells HepG2 with stable expression of 1.1-mer HBV genome were transfected with vectors encoding DNA-methyltransferase 1 (DNMT1), DNA-methyltransferase 3A (DNMT3A) or were co-transfected with these vectors. Total HBV DNA copy number, relative expression of pregenomic RNA (pgRNA), S-protein-encoding RNA (S-RNA) and cccDNA were analyzed by quantitative and semi-quantitative real-time PCR-analysis with TaqMan probes for assessment of DNMTs-mediated effects on HBV. Results: DNMT1 and DNMT3A suppress HBV transcription and replication, though to different magnitude. cccDNA pool is enlarged statistically significantly ≈2-fold (P<0.005) after transfection of DNMT3A, but is unaltered under DNMT1 treatment. Conclusion: DNMT3A regulates the size of cccDNA pool and is important for persistency of HBV infection. Keywords: DNA-methyltransferases (DNMT); PCR; cccDNA; chronic hepatitis B (CHB); hepatitis B virus (HBV); pgRNA.
- Добавил в систему: Костюшев Дмитрий Сергеевич