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Differences in the skin microbiota spectrum and parameters of local immunity in the areas of inflammation in skin diseases and healthy peopleстатья
Статья опубликована в журнале из списка RSCI Web of Science
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из перечня ВАК
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 15 февраля 2024 г.
- Авторы: Sennikova S.V., Toptygina Anna P., Voropaeva E.A.
- Журнал: Российский иммунологический журнал
- Том: 26
- Номер: 4
- Год издания: 2023
- Издательство: ФГБУ "Издательство "Наука"
- Местоположение издательства: Москва
- Первая страница: 477
- Последняя страница: 484
- DOI: 10.46235/1028-7221-13086-dit
- Аннотация: Violation of the microbiota composition is a trigger, and sometimes an etiological factor in the development of chronic skin diseases, such as psoriasis or eczema. Recognition of microflora by keratinocytes and immune cells leads to the production of antimicrobial peptides, chemokines and growth factors, which contributes to the differentiation of T-lymphocytes into autoaggressive effectors of Th1, Th17 and Th22 types that implement autoimmune inflammation in the psoriatic plaque. The aim of the work was to study the differences in the skin microbiota spectrum and the parameters of local immunity in capillary blood taken near the focus of inflammation in patients with autoimmune (psoriasis) and allergic (eczema) diseases compared with the parameters of healthy people. 24 patients with psoriasis (group 1), 20 patients with eczema (group 2) and 20 healthy adults (group 3) were examined. Biological materials were taken (smear with a sterile dry swab into the Amies transport system with activated carbon and capillary blood was taken in 2 microvets, 200 µl each) from the foci of inflammation on the hands skin of patients or from the hands finger of healthy people. Inoculations on diagnostic media, microscopy with Gram staining and identification on a microbiological analyzer were performed. Immunophenotyping of 22 subsets of mononuclear cells was performed by four-color staining of capillary blood with erythrocyte lysis and evaluation of subsets by a flow cytometer. Cytokines in blood plasma were determined by multiplex method. The spectrum of hand skin microflora of the group 3 was more diverse in species composition. In patients with psoriasis and eczema, the coccal flora dominated, with a shift in the microbiota spectrum towards pathobionts. Activation of T and B cells, production of pro-inflammatory cytokines, IL-23/IL-17/IL–22 axis cytokines and cytokines - markers of epithelial cell damage (IL-25 and IL-33), as well as anti-inflammatory factors insufficiency were detected. Differences in changes in the parameters of the local immune status in patients with autoimmune (psoriasis) and allergic (eczema) diseases were revealed, reflecting the features of the immunopathogenesis of these diseases.
- Добавил в систему: Топтыгина Анна Павловна