The Preparation and Properties of Recombinant Forms of GH74  Family Xyloglucanase from the Trichoderma reesei Fungus

Gusakov, A.V.; Klimov, D.A.; Kondratyeva, E.G.; Sinitsyna, O.A.; Rozhkova, A.M.; Volkov, P.V.; Shashkov, I.A.; Sinitsyn, A.P.

Авторы: Gusakov A.V., Klimov D.A., Kondratyeva E.G., Sinitsyna O.A., Rozhkova A.M., Volkov P.V., Shashkov I.A., Sinitsyn A.P.
Журнал: Applied Biochemistry and Microbiology
Том: 59
Номер: 7
Год издания: 2023
Издательство: Maik Nauka/Interperiodica Publishing
Местоположение издательства: Russian Federation
Первая страница: 999
Последняя страница: 1007
DOI: 10.1134/S0003683823070025
Аннотация: Cloning and expression of the full-length endo-processive-type xyloglucanase from the Trichoderma reesei (TrXeg74A) fungus, as well as its catalytic domain TrXeg74A-CD, in the Penicillium verruculosum B1-537 recipient strain have been carried out. P. verruculosum is a highly effective producer of cellulases.The levels of protein secretion after culturing the obtained recombinant strains in a laboratory fermenter were35.4 and 31.4 g/L, respectively. TrXeg74A accounted for at least 30% of the total protein, while TrXeg74A-CD wasexpressed to a much lesser extent. Both forms of the recombinant enzyme were isolated in purified state andtheir properties were studied. TrXeg74A and TrXeg74A-CD were characterized by a similar degree of processivity when exposed to tamarind xyloglucan and the same Michaelis constant (0.35-0.38 g/L), close to thatfor the native enzyme (0.30 g/L), while the catalytic constant for TrXeg74A-CD was 1.5 times higher than thecorresponding parameter for full-length xyloglucanase. The obtained new recombinant P. verruculosumstrains can be useful in the development of composite enzyme preparations for efficient hydrolysis of renewable lignocellulosic raw materials.
Добавил в систему: Синицын Аркадий Пантелеймонович

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The Preparation and Properties of Recombinant Forms of GH74 Family Xyloglucanase from the Trichoderma reesei Fungusстатья

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