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Replicative senescence in human embryo bone marrow MSCs is characterized by changes in cell motility and subcellular localization of Rac1 and RhoA GTPasesстатья
Дата последнего поиска статьи во внешних источниках: 1 мая 2024 г.
- Авторы: Lukacheva A.V., Gorb A.I., Musorina A.S., Kriger D.V., Poljanskaya G.G., Bobkov D.E.
- Журнал: Russian Journal of Bioorganic Chemistry
- Том: 59
- Номер: 1
- Год издания: 2023
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 224
- Последняя страница: 240
- Аннотация: Human mesenchymal stem cells (MSC) are widely used in biomedical technologies, so it is important to study changes in their characteristics that occur during replicative senescence (RS). Assessing cell motility during long-term cultivation is a necessary step for the effective use of MSCs in regenerative medicine. The actin cytoskeleton is constantly being remodeled under the control of Rho family small GTPases, which provide the basis for cell migration. The work aims to investigate human embryonic bone marrow MSCs at different phases of RS. A comparison of the subcellular localization of Rho family small GTPases, cell morphology, and motility was performed during long-term cultivation of the FetMSC line. We examined actin cytoskeleton reorganization and changes in cell motility during RS in the FetMSC line using the activator (LPA) and inhibitor (Y-27632) of the small GTPase RhoA. As a result, along with signs of RS, cell tendencies were found to increase the degree of organization of the actin cytoskeleton and to reduce the nuclear localization of RhoA and Rac1 small GTPases. It was found that the mean speed of FetMSC motility does not decrease during RS, but the sensitivity of cells to the action of Rho signaling modulators changes depending on the passage.
- Добавил в систему: Бобков Данила Евгеньевич
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | 2023_Lukacheva_et_al._RJBC.pdf | 4,3 МБ | 28 декабря 2023 [dan.bobkov] |