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Selective Accumulation of Poly(Lactic-Co-Glycolic Acid) Nanoparticles in Endotheliocytes and Mesenchymal Stromal Cells Cultured as Mixed-Cell Spheroidsстатья
- Авторы: Leonov G.E., Vakhrushev I.V., Novikova V.D., Burunova V.V., Kovshova T.S., Malinovskaya Yu A., Yarygin K.N.
- Журнал: Bulletin of Experimental Biology and Medicine
- Номер: 3
- Год издания: 2024
- Издательство: Springer Nature
- Местоположение издательства: Switzerland
- Первая страница: 241
- Последняя страница: 245
- DOI: 10.1007/s10517-024-06003-4
- Аннотация: The use of drug-loaded nanoparticles is an actively developed approach in targeted cancer therapy. Prevascularized spheroids generated from mesenchymal stem cells and endotheliocytes are considered as a model to evaluate the tropism of therapeutic nanoparticles to a specific tissue. Nanoparticles based on co-polymer of lactic and glycolic acids (poly(lactic-co-glycolic acid; PLGA) labeled with cyanine dye (Cy5) were incubated with prevascularized spheroids, and the rate of their penetration and their distribution in the spheroid-forming cells were evaluated. Endotheliocytes more intensively accumulated nanoparticles than mesenchymal stem cells: the number of nanoparticles in mixed-cell spheroids of mesenchymal stem cells and endotheliocytes was greater than in spheroids built solely of mesenchymal stem cells by 5±1.2 times. The developed 3D in vitro cell model provides a low-cost way to assess tissue tropism of therapeutic nanoparticles under conditions closer to natural in comparison with 2D culture.
- Добавил в систему: Ковшова Татьяна Сергеевна