Spectral and conformational characteristics of phycocyanin associated with changes of medium pH

Авторы: Parshina E.Yu, Liu W., Yusipovich A.I., Gvozdev D.A., He Y., Pirutin S.K., Klimanova E.A., Maksimov E.G., Maksimov G.V.
Журнал: Photosynthesis Research
Год издания: 2024
Издательство: Springer Nature
Местоположение издательства: Switzerland
DOI: 10.1007/s11120-023-01068-0
Аннотация: C-phycocyanin (C-PC) is the main component of water-soluble light-harvesting complexes (phycobilisomes, PBS) of cyanobacteria. PBS are involved in the absorption of quantum energy and the transfer of electronic excitation energy to the photosystems. A specific environment of C-PC chromophoric groups is provided by the protein matrix structure including protein–protein contacts between different subunits. Registration of C-PC spectral characteristics and the fluorescence anisotropy decay have revealed a significant pH influence on the chromophore microenvironment: at pH 5.0, a chromophore is more significantly interacts with the solvent, whereas at pH 9.0 the chromophore microenvironment becomes more viscous. Conformations of chromophores and the C-PC protein matrix have been studied by Raman and infrared spectroscopy. A decrease in the medium pH results in changes in the secondary structure either the C-PC apoproteins and chromophores, the last one adopts a more folded conformation.
Добавил в систему: Юсипович Александр Иванович

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