Аннотация:The discovery of the bioluminescence pathway in the fungus Neonothopanusnambi enabled engineering of eukaryotes with self-sustained luminescence.However, the brightness of luminescence in heterologous hosts was limitedby performance of the native fungal enzymes. Here we report optimizedversions of the pathway that enhance bioluminescence by one to two ordersof magnitude in plant, fungal and mammalian hosts, and enable longitudinalvideo-rate imaging.