|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ИНХС РАН |
||
A hybrid pathway for self-sustained luminescenceстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Palkina Kseniia A., Karataeva Tatiana A., Perfilov Maxim M., Fakhranurova Liliia I., Markina Nadezhda M., Somermeyer Louisa Gonzalez, Garcia-Perez Elena, Vazquez-Vilar Marta, Rodriguez-Rodriguez Marta, Vazquez-Vilriales Victor, Shakhova Ekaterina S., Mitiouchkina Tatiana, Belozerova Olga A., Kovalchuk Sergey I., Alekberova Anna, Malyshevskaia Alena K., Bugaeva Evgenia N., Guglya Elena B., Balakireva Anastasia, Sytov Nikita, Bezlikhotnova Anastasia, Boldyreva Daria I., Babenko Vladislav V., Kondrashov Fyodor A., Choob Vladimir V., Orzaez Diego, Yampolsky Ilia V., Mishin Alexander S., Sarkisyan Karen S.
- Журнал: Science Advances
- Том: 10
- Номер: 10
- Год издания: 2024
- DOI: 10.1126/sciadv.adk1992
- Аннотация: The fungal bioluminescence pathway can be reconstituted in other organisms allowing luminescence imaging without exogenously supplied substrate. The pathway starts from hispidin biosynthesis—a step catalyzed by a large fungal polyketide synthase that requires a posttranslational modification for activity. Here, we report identification of alternative compact hispidin synthases encoded by a phylogenetically diverse group of plants. A hybrid bioluminescence pathway that combines plant and fungal genes is more compact, not dependent on availability of machinery for posttranslational modifications, and confers autonomous bioluminescence in yeast, mammalian, and plant hosts. The compact size of plant hispidin synthases enables additional modes of delivery of autoluminescence, such as delivery with viral vectors.
- Добавил в систему: Чуб Владимир Викторович