Acrylate reductase of an anaerobic electron transport chain of the marine bacterium Shewanella woodyi

Bertsova, Y.V.; Serebryakova, M.V.; Bogachev, V.A.; Baykov, A.A.; Bogachev, A.V.

Авторы: Bertsova Y.V., Serebryakova M.V., Bogachev V.A., Baykov A.A., Bogachev A.V.
Журнал: Biochemistry (Moscow)
Том: 89
Номер: 4
Год издания: 2024
Издательство: Pleiades Publishing, Ltd
Местоположение издательства: Road Town, United Kingdom
Первая страница: 701
Последняя страница: 710
DOI: 10.1134/S0006297924040096
Аннотация: Many microorganisms are capable of anaerobic respiration in the absence of oxygen, by using different organic compounds as terminal acceptors in electron transport chain. We identify here an anaerobic respiratory chain protein responsible for acrylate reduction in the marine bacterium Shewanella woodyi. When the periplasmic proteins of S. woodyi were separated by ion exchange chromatography, acrylate reductase activity copurified with an ArdA protein (Swoo_0275). Heterologous expression of S. woodyi ardA gene (swoo_0275) in Shewanella oneidensis MR-1 cells did not result in the appearance in them of periplasmic acrylate reductase activity, but such activity was detected when the ardA gene was co-expressed with an ardB gene (swoo_0276). Together, these genes encode flavocytochrome c ArdAB, which is thus responsible for acrylate reduction in S. woodyi cells. ArdAB was highly specific for acrylate as substrate and reduced only methacrylate (at a 22-fold lower rate) among a series of other tested 2-enoates. In line with these findings, acrylate and methacrylate induced ardA gene expression in S. woodyi under anaerobic conditions, which was accompanied by the appearance of periplasmic acrylate reductase activity. ArdAB-linked acrylate reduction supports dimethylsulfoniopropionate-dependent anaerobic respiration in S. woodyi and, possibly, other marine bacteria.
Добавил в систему: Богачев Александр Валерьевич

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Acrylate reductase of an anaerobic electron transport chain of the marine bacterium Shewanella woodyiстатья Исследовательская статья

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