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Modular Nanotransporters Capable of Causing Intracellular Degradation of the N-Protein of the SARS-CoV-2 Virus in A549 Cells with Temporary Expression of This Protein Fusedwith a Fluorescent Protein mRuby3статья
Дата последнего поиска статьи во внешних источниках: 15 мая 2024 г.
- Авторы: Khramtsov Y.V., Ulasov A.V., Lupanova T.N., Georgiev G.P., Sobolev A.S.
- Журнал: Doklady Biochemistry and Biophysics
- Том: 513
- Номер: 1
- Год издания: 2023
- Издательство: M A I K Nauka - Interperiodica
- Местоположение издательства: Russian Federation
- Первая страница: S60
- Последняя страница: S62
- DOI: 10.1134/S1607672923700710
- Аннотация: Modular nanotransporters (MNTs) containing an antibody-like molecule, monobody, to theN-protein of the SARS-CoV-2 virus, as well as an amino acid sequence that recruits the Keap1 E3 ligase (E3BP) were created. This MNT also included a site for cleavage of the E3BP monobody from the MNT in acidic endocytic compartments. It was shown that this cleavage by the endosomal protease cathepsin B leads to a 2.7-fold increase in the affinity of the E3BP monobody for the N-protein. Using A549 cells with transient expression of the N-protein fused with the fluorescent protein mRuby3, it was shown that incubation withMNT leads to a significant decrease in mRuby3 fluorescence. It is assumed that the developed MNTs can serve as a basis for the creation of new antiviral drugs against the SARS-CoV-2 virus.
- Добавил в систему: Соболев Александр Сергеевич