Characteristics of the Follicular Fluid Extracellular Vesicle Molecular Profile in Women in Different Age Groups in ART Programs

Sysoeva, A.; Akhmedova, Z.; Nepsha, O.; Makarova, N.; Silachev, D.; Shevtsova, Y.; Goryunov, K.; Karyagina, V.; Bugrova, A.; Starodubtseva, N.; Novoselova, A.; Chagovets, V.; Kalinina, E.

Авторы: Sysoeva Anastasia, Akhmedova Zumriyat, Nepsha Oksana, Makarova Natalya, Silachev Denis, Shevtsova Yulia, Goryunov Kirill, Karyagina Victoria, Bugrova Anna, Starodubtseva Natalya, Novoselova Anastasia, Chagovets Vitaliy, Kalinina Elena
Журнал: Life
Том: 14
Номер: 5
Год издания: 2024
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 541
Последняя страница: 541
DOI: 10.3390/life14050541
Аннотация: The aim of this study was to investigate the molecular composition of follicular fluid (FF) extracellular vesicles (EVs) in women of different reproductive ages and its possible relationship to sperm fertilizing ability. FF EVs were obtained by differential centrifugation. The concentration and size distribution of FF EVs were analyzed by nanoparticle tracking analysis. The lipidome and proteome were analyzed by liquid chromatography–mass spectrometry. The isolated FF EVs had a variety of shapes and sizes; their concentration and size distribution did not differ significantly between the age groups. In women younger than 35 years, the concentration of vesicular progesterone was 6.6 times higher than in women older than 35 years, and the total levels of the main lipid classes were increased in younger women. A proteomic analysis revealed that not only FF EV-specific proteins, but also proteins involved in sperm activation were present. New data were obtained on the composition of FF EVs, confirming their importance as molecular indicators of age-related changes in the female reproductive system. In addition, these results shed light on the possible interaction between the FF EVs of women in different age groups and male germ cells. Therefore, studying the transcriptomic and metabolomic profile of FF EVs may be a crucial approach to evaluate the efficacy of ART.
Добавил в систему: Силачёв Денис Николаевич

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Characteristics of the Follicular Fluid Extracellular Vesicle Molecular Profile in Women in Different Age Groups in ART Programsстатья Исследовательская статья

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