Fluorescence saturation imaging microscopy: molecular fingerprinting with a standard confocal microscope

Yakimov, B.; Rovnyagina, N.; Hasan, A.; Zhang, J.; Wang, H.; Fadeev, V.; Urusova, L.; Khoroshilov, E.; Sharkov, A.; Mokrysheva, N.; Shirshin, E.

Авторы: Yakimov Boris, Rovnyagina Natalia, Hasan Afraa, Zhang Juntao, Wang Haibo, Fadeev Victor, Urusova Liliya, Khoroshilov Evgeny, Sharkov Andrey, Mokrysheva Natalia, Shirshin Evgeny
Журнал: Biomedical optics express
Том: 15
Номер: 6
Год издания: 2024
Издательство: Optical Society of America
Местоположение издательства: United States
Первая страница: 3755
Последняя страница: 3769
DOI: 10.1364/boe.512188
Аннотация: Molecular specificity in fluorescence imaging of cells and tissues can be increasedby measuring parameters other than intensity. For instance, fluorescence lifetime imagingbecame a widespread modality for biomedical optics. Previously, we suggested using thefluorescence saturation effect at pulsed laser excitation to map the absorption cross-section asan additional molecular contrast in two-photon microscopy [Opt. Lett. 47, 4455 (2022)].Here, it is shown that, somewhat counterintuitive, fluorescence saturation can be observedunder cw excitation in a standard confocal microscopy setup. Mapping the fluorescencesaturation parameter allows obtaining additional information about the fluorophores in thesystem, as demonstrated by the example of peptide hydrogel, stained cells and unstainedthyroid gland. The suggested technique does not require additional equipment and can beimplemented on confocal systems as is.
Добавил в систему: Ширшин Евгений Александрович

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Fluorescence saturation imaging microscopy: molecular fingerprinting with a standard confocal microscopeстатья

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