MICROBIOME OF SOILS AND SUPRAGLACIAL OBJECTS IN BACKGROUND AND OIL-POLLUTED ECOSYSTEMS OF HAYES ISLAND, FRANZ JOSEF LAND

Nikitin, D.A.; Manucharova, N.A.; Dobryansky, A.S.

Авторы: Nikitin D.A., Manucharova N.A., Dobryansky A.S.
Журнал: Eurasian Soil Science
Том: 57
Номер: 2
Год издания: 2024
Издательство: Maik Nauka/Interperiodica Publishing
Местоположение издательства: Russian Federation
Первая страница: 251
Последняя страница: 265
Оригинальные версии: МИКРОБИОМ ПОЧВ И СУПРАГЛЯЦИАЛЬНЫХ ОБЪЕКТОВ В ФОНОВЫХ И НЕФТЕЗАГРЯЗНЕННЫХ ЭКОСИСТЕМАХ О. ХЕЙСА (АРХИПЕЛАГ ЗЕМЛЯ ФРАНЦА-ИОСИФА)
DOI: 10.1134/S1064229323602767
Аннотация: The microbiome of soils and supraglacial formations in background and oil-polluted ecosystemsof Hayes Island (Franz Josef Land) was studied using the methods of luminescent microscopy and real-timepolymerase chain reaction. The biomass of microorganisms ranged from 81 to 666 μg C/g substrate; its largerpart (up to 88%) was represented by fungi. The length of the fungal mycelium reached more than 360 m/gsubstrate. The number of prokaryotes varied from 4.0 × 107 to 3.75 × 109 cells/g substrate; the length of actinomycete hyphae reached 40 m/g substrate. Up to 78% of detected prokaryotic cells were represented bysmall nanoforms, which is typical for extreme ecosystems. The proportion of viable microbial cells gainedmaximum (74–86%) in surface organic horizons and minimum (29–54%) in mineral supra-permafrost layers. Bacteria dominated in the prokaryotic complex (from 5.14 × 105 to 5.05 × 1010 16S rRNA copies/g soil);the content of archaea was lower: from 8.46 × 105 to 2.28 × 109 16S rRNA copies/g substrate. The amount offungal ITS rRNA in the soil samples ranged from 6.47 × 104 to 8.67 × 1010 copies. The number of copies ofthe alkB gene (synthesis of alkane monooxygenase for the destruction of n-alkanes of hydrocarbons) variedfrom 1.2 × 101 to 1.8 × 105 copies/g substrate and sharply decreased from surface to deep horizons. Oil-contaminated soils and supraglacial objects contained a smaller biomass, but a larger number of ribosomal genesof microorganisms as compared with background ecosystems. An exponential decrease in the analyzed quantitative parameters of microorganisms from surface to deep soil horizons was observed
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MICROBIOME OF SOILS AND SUPRAGLACIAL OBJECTS IN BACKGROUND AND OIL-POLLUTED ECOSYSTEMS OF HAYES ISLAND, FRANZ JOSEF LANDстатья Исследовательская статья

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