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Antimicrobial Activity of Specialized Metabolites of Soil Chitinolytic Streptomycetesстатья Исследовательская статья
Дата последнего поиска статьи во внешних источниках: 26 июня 2024 г.
- Авторы: Teregulova G.A., Manucharova N.A., Urazbakhtina N.A., Zhemchuzhina N.S., Yevtushenko L.I., Stepanov A.L.
- Журнал: Moscow University Soil Science Bulletin
- Том: 79
- Номер: 1
- Год издания: 2024
- Издательство: Allerton Press Inc.
- Местоположение издательства: United States
- Первая страница: 47
- Последняя страница: 55
- Оригинальные версии: АНТИМИКРОБНАЯ АКТИВНОСТЬ СПЕЦИАЛИЗИРОВАННЫХ МЕТАБОЛИТОВ ПОЧВЕННЫХ СТРЕПТОМИЦЕТОВ-ХИТИНОЛИТИКОВ
- DOI: 10.3103/S0147687424010083
- Аннотация: One-hundred-and-fifty strains of streptomycetes have been isolated from gray and dark gray forestsoils, as well as from typical chernozem. The isolated strains were analyzed in vitro for antimicrobial activityon nutrient media and in gray forest soil against 23 collection pathogenic test cultures of fungi and bacteria.Four biologically active isolates with a wide action spectrum have been identified and deposited at the AllRussia Collection of Industrial Microorganisms of the National Research Center “Kurchatov Institute”under the following numbers: Streptomyces xiamenensis TB VKPM As-2204, Streptomyces anulatus TGVKPM As-2203, Streptomyces sindenensis TK VKPM As-2205, and Streptomyces flavovirens TT VKPM As2202. A study of the effect of presowing treatment of wheat seeds with 15-day culture liquids of S. xiamenensisTB VKPM As-2204, S. anulatus TG VKPM As-2203, and S. sindenensis TK VKPM As-2205 for germinationrates and levels of infection with F. graminearum has revealed that they inhibited the growth of a pathogenicfungus and improved the seed vigor and germination of wheat. The resulting strains of soil actinomycetes canbe used in biotechnology for creating new bioinoculants to combat phytopathogenic bacteria and fungi.The strains can also be used for stimulating the plant growth, as well as for soil bioremediation in organicfarming. Based on the HPLC method (high-performance liquid chromatography), we have identified specialized antimicrobial metabolites of monosporous strain suspensions. The identified antibiotics are N-Butylbenzenesulfonamide, 1-(1H-Benzo[d]imidazol-2-yl)ethan-1-ol, 2-[(3S)-1-(Cyclohexylmethyl)-3-pyrrolidinyl]-1H-benzimidazole-5-carbonitrile, Cyclo(leucylprolyl), and Cyclo(phenylalanyl-prolyl). The identified antiseptics are Cetrimonium and Carvone. The identified phytohormone is auxin indole-3-acetic acid(IAA). Observation of the dynamics of development of the introduced actinobacteria in soil samples hasshown a high activity of streptomycetes that use chitin. Analysis of the diversity of the prokaryotic complex ofthe studied soil samples based on high-throughput sequencing of the conserved region of the 16S rRNA genehas revealed its controlling role in the microbial community during the introduction of S. xiamenensis TBVKPM As-2204, which is determined by its antibiotic-forming activity.
- Добавил в систему: Манучарова Наталия Александровна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | Полный текст | Moscow_University_Soil_Science_Bulletin_1_2024.pdf | 274,8 КБ | 16 мая 2024 [N.A.Manucharova] |
| 2. | Разрешение на публикацию | Vestnik_1_2024_razreshenie.pdf | 200,7 КБ | 16 мая 2024 [N.A.Manucharova] |