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Identification of Chimeric NTRK3 Genes in Papillary Thyroid Cancer Cells by Analyzing the Imbalance of the Expression of 5' and 3' mRNA Fragmentsстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 29 мая 2024 г.
- Авторы: Оскорбин И.П., Иванов А.А., Смертина М.А., Демидова И.А., Боярских У.А., Кечин А.А., Бахарев С.Ю., Самуйленкова О.В., Вихлянов И.В., Кушлинский Н.Е., Филипенко М.Л.
- Журнал: Bulletin of Experimental Biology and Medicine
- Том: 175
- Год издания: 2023
- Издательство: Springer Nature
- Местоположение издательства: Switzerland
- Первая страница: 239
- Последняя страница: 244
- DOI: 10.1007/s10517-023-05842-x
- Аннотация: The standard for detecting chimeric genes of neurotrophic receptor tyrosine kinases (NTRK) is next generation sequencing (NGS). However, this analysis is expensive and takes several days. As a rapid screening method for the detection of NTRK3-dependent papillary thyroid cancer, an analysis of the expression imbalance between 5’ and 3’ NTRK3 mRNA fragments was used (5’/3’ RT-PCR). The reference method for detection of NTRK3 rearrangements was fluorescent in situ hybridization (FISH), and the most frequent rearrangements in papillary thyroid cancer were tested using reverse transcription PCR (RT-PCR). Using 5’/3’ RT-PCR, 18 samples of papillary thyroid cancer carrying chimeric transcripts of NTRK3 mRNA were detected. The sensitivity of the developed technique was 88.9% and specificity was 99.3%. Thus, a fast and cost-effective method of screening samples of papillary thyroid cancer in paraffin blocks is proposed with acceptable sensitivity and specificity.
- Добавил в систему: Кубасова Ирина Юрьевна