To the novel paradigm of proteome-based cell therapy of tumors: through comparative proteome mapping of tumor stem cells and tissue-specific stem cells of humans

Bryukhovetskiy, A.; Shevchenko, V.; Kovalev, S.; Chekhonin, V.; Baklaushev, V.; Bryukhovetskiy, I.; Zhukova, M.

Авторы: Bryukhovetskiy Andrey, Shevchenko Valeriy, Kovalev Sergey, Chekhonin Vladimir, Baklaushev Vladimir, Bryukhovetskiy Igor, Zhukova Maria
Журнал: Cell Transplantation
Том: 23
Номер: 1
Год издания: 2014
Издательство: Cognizant Communication Corp.
Местоположение издательства: United States
Первая страница: 151
Последняя страница: 170
DOI: 10.3727/096368914X684907
Аннотация: We performed proteome mapping (PM), cataloging, and bioinformation analysis of protein lysates of human neural (CD133+) progenitor and stem cells (NPSCs) isolated from the olfactory sheath of a nose, multipotent mesenchymal (CD29+, CD44+, CD73+, CD90+, CD34−) stromal cells (MMSCs) isolated from human bone marrow, and tumor (CD133+) stem cells (TSCs) isolated from the human U87 glioblastoma (GB) cell line. We identified 1,664 proteins in the examined lysates of stem cells (SCs), 1,052 (63.2%) of which are identical in NPSCs and TSCs and 607 proteins (36.47%) of which are identical in MMSCs and TSCs. Other proteins in U87 GB TSCs are oncospecific or carcinogenesis associated. The biological processes, molecular functions, cell localization, and protein signal pathways of the proteins available in all three proteomes were annotated by PubMed (http://www.ncbi.nlm.nih.gov/pubmed/), PANTHER (http://www.pantherdb.org/), GeneOntology (http://www.geneontology.org/), and KEGG (http://www.genome.jp/kegg/) databases. It was shown that gliomaspheres of U87 GB had only 10 intracellular signal transduction pathways (ISTP) that were not modified by the neoplastic process, but only two of them (integrin and focal adhesion pathways) were accessible for regulatory action on gene candidates in the TSC nucleus. Carcinogenesis-free membrane proteins, IPST, and genes expressing proteins of these pathways in U87 GB TSCs can be viewed as main targets for regulatory effects on TSCs. We offer a novel concept of proteome-based complex therapy of tumors. This manuscript is published as part of the International Association of Neurorestoratology (IANR) special issue of Cell Transplantation.
Добавил в систему: Ковалев Сергей Васильевич

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To the novel paradigm of proteome-based cell therapy of tumors: through comparative proteome mapping of tumor stem cells and tissue-specific stem cells of humansстатья

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