Multifunctional Biosensor based on Localized Surface Plasmon Resonance for Monitoring Small Molecule-Protein Interactionстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 2 октября 2014 г.
Аннотация:We report an optical sensor based on localized Surface Plasmon Resonance (LSPR) to study small-molecule protein interaction combining high sensitivity refractive index sensing for quantitative binding information and subsequent conformation-sensitive plasmon-activated Circular Dichroism spectroscopy. The interaction of alpha-amylase and a small-size molecule (PGG - pentagalloyl glucose) was log concentration-dependent from 0.5 to 154 µM. In situ tests were additionally successfully applied to the analysis of real wine samples. These studies demonstrate that LSPR sensors to monitor small-molecules-proteins interactions in real time and in situ which is a great advance within technological platforms for drug discovery.