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Analytical Methods for Assessing Thiol Antioxidants in Biological Fluids: A Reviewстатья Исследовательская статья
- Авторы: Poimenova Iuliia A., Sozarukova Madina M., Ratova Daria-Maria V., Nikitina Vita N., Khabibullin Vladislav R., Mikheev Ivan V., Proskurnina Elena V., Proskurnin Mikhail A.
- Журнал: Molecules
- Том: 29
- Номер: 18
- Год издания: 2024
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- DOI: 10.3390/molecules29184433
- Аннотация: Redox metabolism is an integral part of the glutathione system, encompassing reduced and oxidized glutathione, hydrogen peroxide, and associated enzymes. This core process orchestrates a network of thiol antioxidants like thioredoxins and peroxiredoxins, alongside critical thiol-containing proteins such as mercaptoalbumin. Modifications to thiol-containing proteins, including oxidation and glutathionylation, regulate cellular signaling influencing gene activities in inflammation and carcinogenesis. Analyzing thiol antioxidants, especially glutathione, in biological fluids offers insights into pathological conditions. This review discusses the analytical methods for biothiol determination, mainly in blood plasma. The study includes all key methodological aspects of spectroscopy, chromatography, electrochemistry, and mass spectrometry, highlighting their principles, benefits, limitations, and recent advancements that were not included in previously published reviews. Sample preparation and factors affecting thiol antioxidant measurements are discussed. The review reveals that the choice of analytical procedures should be based on the specific requirements of the research. Spectrophotometric methods are simple and cost-effective but may need more specificity. Chromatographic techniques have excellent separation capabilities but require longer analysis times. Electrochemical methods enable real-time monitoring but have disadvantages such as interference. Mass spectrometry-based approaches have high sensitivity and selectivity but require sophisticated instrumentation. Combining multiple techniques can provide comprehensive information on thiol antioxidant levels in biological fluids, enabling clearer insights into their roles in health and disease. This review covers the time span from 2010 to mid-2024, and the data were obtained from the SciFinder® (ACS), Google Scholar (Google), PubMed®, and ScienceDirect (Scopus) databases through a combination search approach using keywords.
- Добавил в систему: Михеев Иван Владимирович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | Полный текст | molecules-29-04433-v2.pdf | 2,0 МБ | 18 ноября 2024 [nikivita] |