Bacteroides vesicles promote functional alterations in the gut microbiota composition

Shagaleeva, O.Y.; Kashatnikova, D.A.; Kardonsky, D.A.; Efimov, B.A.; Ivanov, V.A.; Smirnova, S.V.; Evsiev, S.S.; Zubkov, E.A.; Abramova, O.V.; Zorkina, Y.A.; Morozova, A.Y.; Vorobeva, E.A.; Silantiev, A.S.; Kolesnikova, I.V.; Markelova, M.I.; Olekhnovich, E.I.; Morozov, M.D.; Zoruk, P.Y.; Boldyreva, D.I.; Kazakova, V.D.; Vanyushkina, A.A.; Chaplin, A.V.; Grigoryeva, T.V.; Zakharzhevskaya, N.B.

Авторы: Shagaleeva Olga Yu, Kashatnikova Daria A., Kardonsky Dmitry A., Efimov Boris A., Ivanov Viktor A., Smirnova Svetlana V., Evsiev Suleiman S., Zubkov Eugene A., Abramova Olga V., Zorkina Yana A., Morozova Anna Y., Vorobeva Elizaveta A., Silantiev Artemiy S., Kolesnikova Irina V., Markelova Maria I., Olekhnovich Evgenii I., Morozov Maxim D., Zoruk Polina Y., Boldyreva Daria I., Kazakova Victoriia D., Vanyushkina Anna A., Chaplin Andrei V., Grigoryeva Tatiana V., Zakharzhevskaya Natalya B.
Журнал: Microbiology Spectrum
Год издания: 2024
DOI: 10.1128/spectrum.00636-24
Аннотация: Inflammatory bowel diseases are characterized by chronic intestinal inflammation and alterations in the gut microbiota composition. Bacteroides fragilis, which secretes outer membrane vesicles (OMVs) with polysaccharide A (PSA), can moderate the inflammatory response and possibly alter the microbiota composition. In this study, we created a murine model of chronic sodium dextran sulfate (DSS)-induced intestinal colitis and treated it with B. fragilis OMVs. We monitored the efficiency of OMV therapy by determining the disease activity index (DAI) and performing histological examination (HE) of the intestine before and after vesicle exposure. We also analyzed the microbiota composition using 16S rRNA gene sequencing. Finally, we evaluated the volatile compound composition in the animals’ stools by HS-GC/MS to assess the functional activity of the microbiota. We observed more effective intestinal repair after OMV treatment according to the DAI and HE. A metabolomic study also revealed changes in the functional activity of the microbiota, with a predominance of phenol and pentanoic acid in the control group compared to the group treated with DSS and the group treated with OMVs (DSS OMVs). We also observed a positive correlation of these metabolites with Saccharibacteria and Acetivibrio in the control group, whereas in the DSS group, there was a negative correlation of phenol and pentanoic acid with Lactococcus and Romboutsia. According to the metabolome and sequencing data, the microbiota composition of the DSS-treated OMV group was intermediate between that of the control and DSS groups. OMVs not only have an anti-inflammatory effect but also contribute to the recovery of the microbiota composition.
Добавил в систему: Воробьева Елизавета Андреевна

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Bacteroides vesicles promote functional alterations in the gut microbiota compositionстатья