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Physiological and Ultrastructural Responses of Nostoc sp. (Cyanobacteriota) Strains to Phosphorus Starvation under Non-Diazotrophic Conditionsстатья
- Авторы: Scherbakov P.N., Selyakh I.O., Semenova L.R., Chivkunova O.B., Baulina O.I., Karpova O.V., Lobakova E.S., Solovchenko A.E., Gorelova O.A.
- Журнал: Microbiology
- Том: 93
- Номер: 6
- Год издания: 2024
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 812
- Последняя страница: 825
- DOI: 10.1134/S0026261724605359
- Аннотация: Phosphorus (P) is an essential macronutrient central to the exchange and storage of energy andinformation in the cell. Due to its limited bioavailability, P often becomes a limiting nutrient in aquatic andterrestrial ecosystems hence the studies of responses to stress caused by P starvation cyanobacteria, the pri-mary producers, are of considerable interest. Indeed, the availability of P is among main factors limiting diaz-otrophy in cyanobacteria. To gain a deeper understanding of the effect of P starvation on cyanobacteria innon-diazotrophic conditions, we studied a model system of two near—isogenic strains, Nostoc sp. PCC 7120and Nostoc sp. PCC 7118 differing in their ability to form heterocysts. Specifically, we investigated the differ-ences in the responses of these strains to P starvation by comparing their growth kinetics, photosynthetic pig-ment content, ultrastructural rearrangements of vegetative cells, and the expression profile of key genes ofphosphorus metabolism. The tolerance of PCC 7120 to P starvation was higher than that of PCC 7118, whichmanifested itself in a higher growth rate, less profound ultrastructural changes (in particular, phycobilisomesas well as polyphosphate reserves were retained in the cells of PCC 7120). At the same time, the accumulationof cyanophycin, a depot of nitrogen and energy, increased several-fold in the cells of both strains during Pstarvation, but this increase was larger in PCC 7118 cells. Assumably, the increased resilience of the PCC 7120to P starvation stems from its higher ability to accumulate intracellular reserves of P in the form of polyphos-phates. Our findings suggest that the phenotypic differences between the strains Nostoc sp. PCC 7118 andNostoc sp. PCC 7120 are not limited to the different ability to form heterocysts. A deeper understanding of thedrivers of stress response phenotypic diversity in near-isogenic strains would require a comparative analysisof their whole-genome sequences.
- Добавил в систему: Соловченко Алексей Евгеньевич
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | Scherbakov_et_all_MCRO812.pdf | 2,4 МБ | 21 ноября 2024 [Lmnoppi] | |
| 2. | Экспертное заключение | Expert_conclusion_Scherbakov_et_all.pdf | 3,1 МБ | 18 ноября 2024 [Lmnoppi] |