Differences in expression and metabolomic responses in garlic (Allium sativum L.) cultivars, contrasting in Fusarium rot resistance, to Fusarium proliferatum infectionтезисы доклада
Аннотация:Fungi of the genus Fusarium are the most harmful garlic pathogens, causing bulb rot and leaf wilt.In this work, for the first time, genes of various families related to the biotic stress response were identified in the A. sativum genome and characterized: Pathogenesis-Related (PR1, PR2, PR3, PR4, and PR5 families), flavin-containing monooxygenases (FMO), monodehydroascorbate reductases (MDHAR), soluble sugar uniporters (SWEET) and DREB transcription factors. In garlic cv. Sarmat and Strelets, contrasting in Fusarium rot resistance, the expression response of the studied gene families to F. proliferatum infection was determined. It was found that in the resistant cv. Sarmat, the expression of most of the genes is significantly (100-3000 times) upregulated by the infection, while in the susceptible cv. Strelets, gene expression does not change or significantlydecreases. A number of DEGs were characterized in terms of coding and regulatory sequence variability. Metabolome profiling showed a change in the content of organosulfur compounds in garlic roots in response to F. proliferatum infection.This work was supported by the RSF grant no. 21-76-00007.