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On the Properties of Styrene–Maleic Acid Copolymer–Lipid Nanoparticles: A Solution NMR Perspectiveстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Motov Vladislav V., Kot Erik F., Kislova Svetlana O., Bocharov Eduard V., Arseniev Alexander S., Boldyrev Ivan A., Goncharuk Sergey A., Mineev Konstantin S.
- Журнал: Polymers
- Том: 16
- Номер: 21
- Год издания: 2024
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Номер статьи: 3009
- DOI: 10.3390/polym16213009
- Аннотация: The production of functionally active membrane proteins (MPs) in an adequate membrane environment is a key step in structural biology. Polymer–lipid particles based on styrene and maleic acid (SMA) represent a promising type of membrane mimic, as they can extract properly folded MPs directly from their native lipid environment. However, the original SMA polymer is sensitive to acidic pH levels, which has led to the development of several modifications: SMA-EA, SMA-QA, and others. Here, we introduce a novel SMA derivative with a negatively charged taurine moiety, SMA-tau, and investigate the formation and characteristics of lipid–SMA-EA and lipid–SMA-tau membrane-mimicking particles. Our findings demonstrate that both polymers can form nanodiscs with a patch of lipid bilayer that can undergo phase transitions at temperatures close to those of the lipid bilayer membranes. Finally, we discuss the potential applications of these SMAs for NMR spectroscopy.
- Добавил в систему: Болдырев Иван Александрович