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Human TRPV1 is an efficient thermogenetic actuator for chronic neuromodulationстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Maltsev Dmitry I., Solotenkov Maxim A., Mukhametshina Liana F., Sokolov Rostislav A., Solius Georgy M., Jappy David, Tsopina Aleksandra S., Fedotov Ilya V., Lanin Aleksandr A., Fedotov Andrei B., Krut’ Viktoriya G., Ermakova Yulia G., Moshchenko Aleksandr A., Rozov Andrei, Zheltikov Aleksei M., Podgorny Oleg V., Belousov Vsevolod V.
- Журнал: Cellular and Molecular Life Sciences
- Том: 81
- Номер: 1
- Год издания: 2024
- Издательство: Birkhauser Verlag
- Местоположение издательства: Switzerland
- Номер статьи: 437
- DOI: 10.1007/s00018-024-05475-x
- Аннотация: Thermogenetics is a promising neuromodulation technique based on the use of heat-sensitive ion channels. However, on the way to its clinical application, a number of questions have to be addressed. First, to avoid immune response in future human applications, human ion channels should be studied as thermogenetic actuators. Second, heating levels necessary to activate these channels in vivo in brain tissue should be studied and cytotoxicity of these temperatures addressed. Third, the possibility and safety of chronic neuromodulation has to be demonstrated. In this study, we present a comprehensive framework for thermogenetic neuromodulation in vivo using the thermosensitive human ion channel hTRPV1. By targeting hTRPV1 expression to excitatory neurons of the mouse brain and activating them within a non-harmful temperature range with a fiber-coupled infrared laser, we not only induced neuronal firing and stimulated locomotion in mice, but also demonstrated that thermogenetics can be employed for repeated neuromodulation without causing evident brain tissue injury. Our results lay the foundation for the use of thermogenetic neuromodulation in brain research and therapy of neuropathologies.
- Добавил в систему: Ланин Александр Александрович