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Mitochondrial Genome Instability in W303-SK1 Yeast Cytoplasmic Hybridsстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Epremyan Khoren K., Burlaka Arteom A., Markova Olga V., Galkina Kseniia V., Knorre Dmitry A.
- Журнал: Biology
- Том: 13
- Номер: 11
- Год издания: 2024
- Издательство: MDPI Publishing
- Местоположение издательства: Basel, Switzerland, Switzerland
- Первая страница: 927
- Последняя страница: 927
- DOI: 10.3390/biology13110927
- Аннотация: Simple SummaryMitochondrial respiratory chain subunits are encoded by both mitochondrial and nuclear genomes, imposing evolutionary constraints due to potential incompatibilities between mutations in these genomes. Our study demonstrates that swapping mitochondrial DNA (mtDNA) between two laboratory yeast strains increases the frequency of mtDNA loss and reduces their respiratory capacity, suggesting coadaptation of mitochondrial and nuclear genomes at the intraspecific level. We also show that the ability of selfish mtDNA to displace other variants in heteroplasmic yeast cells depends primarily on their mtDNA sequences rather than the nuclear genome background. These findings underscore the complex interactions between mitochondrial and nuclear genomes.AbstractUnlike most animals, some fungi, including baker’s yeast, inherit mitochondrial DNA (mtDNA) from both parents. When haploid yeast cells fuse, they form a heteroplasmic zygote, whose offspring retain one or the other variant of mtDNA. Meanwhile, some mutant mtDNA (rho−), with large deletions in the nucleotide sequence, can displace wild-type (rho+) mtDNA. Consequently, offspring of zygotes with such rho− mtDNA predominantly carry the mutant variant. This phenomenon is called suppressivity. In this study, we investigated how the suppressivity of rho− mtDNA depends on the mitochondrial and nuclear genomes of the rho+ strain during crossing. Comparing two diverged laboratory strains, SK1 and W303, we measured suppressivity in crosses with four rho− strains. One rho− strain showed significantly higher suppressivity when crossed with SK1 than with W303. We then created cytoplasmic hybrids by swapping mtDNAs between these strains. Surprisingly, we found that the mtDNA of the rho+ strain, rather than its nuclear DNA, determines high suppressivity in crosses of SK1 rho+ with the rho− strain. Additionally, mtDNA replacement reduced respiration rate and growth rate on non-fermentable substrates while increasing the likelihood of functional mtDNA loss. Our data demonstrate that a mutant mtDNA variant’s ability to displace another mitochondrial DNA variant in a heteroplasmic cell depends more on mtDNA sequences than on the biochemical and structural context created by the nuclear genome background.
- Добавил в систему: Бурлака Артем Алексеевич