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Antioxidant properties of the soluble carotenoprotein AstaP and its feasibility for retinal protection against oxidative stressстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Lunegova Daria A., Gvozdev Danil A., Senin Ivan I., Gudkova Victoria R., Sidorenko Svetlana V., Tiulina Veronika V., Shebardina Natalia G., Yakovleva Marina A., Feldman Tatiana B., Ramonova Alla A., Moysenovich Anastasia M., Semenov Alexey N., Zernii Evgeni Yu, Maksimov Eugene G., Sluchanko Nikolai N., Kirpichnikov Mikhail P., Ostrovsky Mikhail A.
- Журнал: FEBS Journal
- Том: 292
- Год издания: 2024
- Издательство: Blackwell Publishing Inc.
- Местоположение издательства: United Kingdom
- Первая страница: 355
- Последняя страница: 372
- DOI: 10.1111/febs.17335
- Аннотация: Photodamage to the outer segments of photoreceptor cells and their impaired utilization by retinal pigment epithelium (RPE) cells contribute to the development of age-related macular degeneration (AMD) leading to blindness. Degeneration of photoreceptor cells and RPE cells is triggered by reactive oxygen species (ROS) produced by photochemical reactions involving bisretinoids, by-products of the visual cycle, which accumulate in photoreceptor discs and lipofuscin granules of RPE. Carotenoids, natural antioxidants with high potential efficacy against a wide range of ROS, may protect against the cytotoxic properties of lipofuscin. To solve the problem of high hydrophobicity of carotenoids and increase their bioaccessibility specialized proteins can ensure their targeted delivery to the affected tissues. In this study, we present new capabilities of the recombinant water-soluble protein AstaP from the Coelastrella astaxanthina Ki-4 (Scenedesmaceae) for protein-mediated carotenoid delivery and demonstrate how zeaxanthin delivery suppresses oxidative stress in a lipofuscin-enriched model of photoreceptor and pigment epithelium cells. AstaP in complex with zeaxanthin can effectively scavenge various ROS (singlet oxygen, free radical cations, hydrogen peroxide) previously reported to be generated in AMD. In addition, we explore the potential of optimizing the structure of AstaP to enhance its thermal stability and resistance to proteolytic activity in the ocular media. This optimization aims to maximize the prevention of retinal degenerative changes in AMD.
- Добавил в систему: Сидоренко Светлана Вадимовна
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