Cap-independent translation initiation of apaf-1 mRNA based on a scanning mechanism is determined by some features of the secondary structure of its 5' untranslated region

Andreev, D.E.; Dmitriev, S.E.; Terenin, I.M.; Shatsky, I.N.

Авторы: Andreev D.E., Dmitriev S.E., Terenin I.M., Shatsky I.N.
Журнал: Biochemistry (Moscow)
Том: 78
Номер: 2
Год издания: 2013
Издательство: Pleiades Publishing, Ltd
Местоположение издательства: Road Town, United Kingdom
Первая страница: 157
Последняя страница: 165
Оригинальные версии: Кеп-независимая инициация трансляции по сканирующему механизму у мРНК Apaf-1 определяется особенностями вторичной структуры ее 5' нетранслирумой области
DOI: 10.1134/S0006297913020041
Аннотация: We have earlier shown that the 5¢-untranslated region (5¢ UTR) of the mRNA coding for activation factor of apoptotic peptidase 1 (Apaf-1) can direct translation in vivo by strictly 5¢ end-dependent way even in the absence of m7Gcap. Dependence of translational efficiency on the cap availability for this mRNA turned out to be relatively low. In this study we demonstrate that this surprising phenomenon is determined the 5¢-proximal part (domains I and II) of highly structured Apaf-1 5¢ UTR. Remarkably, domain II by itself was able to reduce dependence of the mRNA on the cap on its transferring to a short 5¢ UTR derived from a standard vector. We suggest that the low cap-dependence inherent to some cellular mRNAs may have an important physiological significance under those stress conditions when the function of capbinding factor eIF4E is impaired.
Добавил в систему: Дмитриев Сергей Евгеньевич

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Cap-independent translation initiation of apaf-1 mRNA based on a scanning mechanism is determined by some features of the secondary structure of its 5' untranslated regionстатья

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