Alkalothermophilic Iron-Reducing Bacteria of the Goryachinsk Thermal Water Basin

Zavarzina, D.G.; Klyukina, A.A.; Merkel, A.Y.; Maslova, T.A.; Maslov, A.A.

Авторы: Zavarzina D.G., Klyukina A.A., Merkel A.Y., Maslova T.A., Maslov A.A.
Журнал: Microbiology
Том: 93
Номер: 6
Год издания: 2024
Издательство: Pleiades Publishing, Ltd
Местоположение издательства: Road Town, United Kingdom
Первая страница: 864
Последняя страница: 875
DOI: 10.1134/S0026261724606845
Аннотация: A study of iron-reducing prokaryotes that use the poorly crystalline mineral ferrihydrite as an electronacceptor revealed their widespread occurrence on the planet. However, the ability of iron reducers todevelop under polyextreme conditions (at elevated values of pH and temperature) has not been previouslydemonstrated. To confirm the existence of alkalothermophilic iron reducers, we studied the water and sedimentsamples from the Goryachinsk nitrogen-rich thermal water basin. These springs are associated withyoung tectonic fractures in the Baikal rift zone; their water is characterized by low salinity, alkalinepH (8.8‒9.2) and temperature of 53‒55°C. Enrichment cultures reducing synthesized ferrihydrite wereobtained by adding this mineral to the water and sediment samples from the Goryachinsk thermal springs,followed by anaerobic incubation at 50 and 55°C. The 16S rRNA gene profiling of the water and sedimentsamples and of the primary enrichment cultures revealed high taxonomic diversity, almost exclusively representedby bacterial taxa. Subsequent transfers with ferrihydrite and organic acids or hydrogen as electrondonors resulted in stable enrichment cultures of organo- and lithotrophic alkalothermophilic iron reducers.Members of the genus Parvivirga, one of the first cultured representatives of the OPB41 group (now the orderAnaerosomatales, phylum Actinomycetota), constituted 30 to 50% of the total abundance.
Добавил в систему: Маслов Алексей Анатольевич

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Alkalothermophilic Iron-Reducing Bacteria of the Goryachinsk Thermal Water Basinстатья

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