Structure and Stability of Anionic Liposomes Complexeswith PEG-Chitosan Branched Copolymerстатья
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Дата последнего поиска статьи во внешних источниках: 12 января 2015 г.
Аннотация:Abstract—Application of branched copolymers of polyethylene glycol and chitosan (PEGchitosan) as sta
bilizing agents for anionic liposomes was shown to improve considerably liposomes storage stability. In the
course of the work, an efficient and convenient approach to synthesis of PEGchitosan copolymers through
chemical modification of chitosan amino groups with monomethoxyPEGNhydroxysuccinimidyl succi
nate (mPEGsucNHS) was developed. Chitosan with varying degree of PEGylation were obtained and used
as stabilizing agents for anionic liposomes prepared of dipalmitoylphosphatidylcholine–cardiolipin, 80/20
by weight. The molecular mechanism of complex formation between the anionic liposomes and PEGchito
san was studied by methods of FTIR spectroscopy and dynamic light scattering. Phosphate and carbonyl
groups were found to be the main sites of the aminopolysaccharide binding. Stabilization of the complexes is
mainly achieved through electrostatic interactions between anionic groups of cardiolipin and free amino
groups of PEGchitosan. The method of liposome stabilization is promising for the development of new drug
delivery systems.
Keywords: anionic liposomes, IR spectroscopy, dynamic light scattering, PEGchitosan, branched copolymers,
stabilization