Аннотация:Inflammatory processes are a typical response of the organism to various injuries, providing elimination of the causes of damage and tissue repair. In the course of cell damage, reactive oxygen species (ROS) are formed, which stimulate further cell destruction. One way to stop the secondary inflammatory process is to use antioxidant enzymes, such as superoxide dismutase, which can neutralize active radicals. However, native enzymes in the body are quickly proteolyzed and eliminated. One of the most effective ways to protect an enzyme in the body is to coat it with polymer complexes. The aim of this study is to synthesize and characterize nanoparticles based on superoxide dismutase (SOD) enzyme encapsulated in cationic block copolymers poly(ethylene glycol)-poly(L-lysine) (PEG-PLL) and N3-poly(ethylene glycol)-poly{N’-[N-(2-aminoethyl)-2-aminoethyl]aspartamide (N3-PEG-pAsp-DET) using the crosslinking agent bis-sulfosuccinimidyl suberate (BS3). It is also planned to study the targeting delivery of nanoparticles using nanoparticle conjugates with E-selectin antibody (CD62E HAE 1-f).
