Physical and chemical analysis of the lipofuscin granule of bisretinoid photodestruction products from retinal pigment epithelium cells of the eye

Yakovleva, M.A.; Vasin, A.A.; Dontsov, A.E.; Gulin, A.A.; Aybush, A.V.; Astafiev, A.A.; Shakhov, A.M.; Feldman, T.B.; Ostrovsky, M.A.

Авторы: Yakovleva M.A., Vasin A.A., Dontsov A.E., Gulin A.A., Aybush A.V., Astafiev A.A., Shakhov A.M., Feldman T.B., Ostrovsky M.A.
Журнал: Russian Journal of Physical Chemistry B
Том: 18
Номер: 6
Год издания: 2024
Издательство: Maik Nauka/Interperiodica Publishing
Местоположение издательства: Russian Federation
Первая страница: 1592
Последняя страница: 1603
DOI: 10.1134/S1990793124701276
Аннотация: In this paper, the mechanisms of the formation of the bisretinoid oxidation products in lipofuscingranules (LGs) isolated from the retinal pigment epithelium cells of the human eye are studied. The physico-chemical characteristics of the bisretinoid photooxidation products are described. The methods of IR spectroscopy, Raman spectroscopy, fluorescence spectroscopy, scanning confocal microscopy, time-of-flight mass spectrometry of secondary ions (TOF.SIMS), and HPLC are used for the study. The properties of the products of photooxidation and degradation of the fluorophore of LGs, including synthetic N-retinylideneN-retinylethanolamine (A2E), are described in detail. It is shown that the products of oxidative degradation of LGs are similar to the products of photooxidation of the main bisretinoid of LGs: A2E. These data are important both for understanding the mechanisms of the formation of cytotoxic products in LGs and for establishing their chemical nature.
Добавил в систему: Фельдман Татьяна Борисовна

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Physical and chemical analysis of the lipofuscin granule of bisretinoid photodestruction products from retinal pigment epithelium cells of the eyeстатья Исследовательская статья

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