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Vic9 mycobacteriophage: the first subcluster B2 phage isolated in Russiaстатья
- Авторы: Zaychikova Marina, Malakhova Maja, Bespiatykh Dmitry, Kornienko Maria, Klimina Ksenia, Strokach Aleksandra, Gorodnichev Roman, German Arina, Fursov Mikhail, Bagrov Dmitry, Vnukova Anna, Gracheva Alexandra, Kazyulina Anastasia, Shleeva Margarita, Shitikov Egor
- Журнал: Frontiers in microbiology
- Год издания: 2025
- Издательство: Frontiers Research Foundation
- Местоположение издательства: Switzerland
- DOI: 10.3389/fmicb.2024.1513081
- Аннотация: Mycobacteriophages are viruses that specifically infect bacteria of the Mycobacteriumgenus. A substantial collection of mycobacteriophages has been isolated andcharacterized, offering valuable insights into their diversity and evolution. Thiscollection also holds significant potential for therapeutic applications, particularlyas an alternative to antibiotics in combating drug-resistant bacterial strains. In thisstudy, we report the isolation and characterization of a new mycobacteriophage,Vic9, using Mycobacterium smegmatis mc (2)155 as the host strain. Vic9 has beenclassified within the B2 subcluster of the B cluster. Morphological analysis revealedthat Vic9 has a structure typical of siphophages from this subcluster and formscharacteristic plaques. The phage adsorbs onto host strain cells within 30 min, andaccording to one-step growth experiments, its latent period lasts about 90 min,followed by a growth period of 150 min, with an average yield of approximately68 phage particles per infected cell. In host range experiments, Vic9 efficientlylysed the host strain and also exhibited the ability to lyse M. tuberculosis H37Rv,albeit with a low efficiency of plating (EOP ≈ 2 × 10−5), a typical feature of B2phages. No lysis was observed in other tested mycobacterial species. The genomeof Vic9 comprises 67,543 bp of double-stranded DNA and encodes 89 openreading frames. Our analysis revealed unique features in Vic9, despite its closerelationship to other B2 subcluster phages, highlighting its distinct characteristicseven among closely related phages. Particularly noteworthy was the discoveryof a distinct 435 bp sequence within the gene cluster responsible for queuosinebiosynthesis, as well as a recombination event within the structural cassette region(Vic_0033-Vic_0035) among members of the B1, B2, and B3 subclusters. Thesegenetic features are of interest for further research, as they may reveal newmechanisms of phage-bacteria interactions and their potential for developingnovel phage therapy methods.KEYWORDSmycobacteriophages, Mycobacterium tuberculosis, host range, B cluster, one-stepgrowth curve
- Добавил в систему: Внукова Анна Александровна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | Vic9 mycobacteriophage: the first subcluster B2 phage isolated in Russia | fmicb-1-1513081.pdf | 2,1 МБ | 14 января 2025 [VnukovaAA] |