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Optimizing FlowCam Imaging Flow Cytometry Operation for Classification and Quantification of Microcystis Morphospeciesстатья
Информация о цитировании статьи получена из
Scopus
Дата последнего поиска статьи во внешних источниках: 5 февраля 2025 г.
- Авторы: Malashenkov D., Dashkova V., Vorobjev IA, Barteneva NS
- Сборник: Spectral and Imaging Cytometry: Methods and Protocols
- Серия: Methods in Molecular Biology
- Том: 2635
- Год издания: 2023
- Издательство: Springer US
- Местоположение издательства: New York, United States
- Первая страница: 245
- Последняя страница: 258
- DOI: 10.1007/978-1-0716-3020-4_14
- Аннотация: Microcystis is a globally known cyanobacterium causing potentially toxic blooms worldwide. Different morphospecies with specific morphological and physiological characters usually co-occur during blooming, and their quantification employing light microscopy can be time-consuming and problematic. A benchtop imaging flow cytometer (IFC) FlowCam (Yokogawa Fluid Imaging Technologies, USA) was used to identify and quantitate different Microcystis morphospecies from environmental samples. We describe here the FlowCam methodology for sample processing and analysis of five European morphospecies of Microcystis common to the temperate zone. The FlowCam technique allows detection of different Microcystis morphospecies providing objective qualitative and quantitative data for statistical analysis.
- Добавил в систему: Малашенков Дмитрий Владимирович