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Structure and dynamics of a nucleosome core particle based on Widom 603 DNA sequenceстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Armeev Grigoriy A., Moiseenko Andrey V., Motorin Nikita A., Afonin Dmitriy A., Zhao Lei, Vasilev Veniamin A., Oleinikov Pavel D., Glukhov Grigory S., Peters Georgy S., Studitsky Vasily M., Feofanov Alexey V., Shaytan Alexey K., Shi Xiangyan, Sokolova Olga S.
- Журнал: Structure
- Том: 33
- Номер: 5
- Год издания: 2025
- Издательство: Cell Press
- Местоположение издательства: United States
- Первая страница: 948
- Последняя страница: 959.e5
- DOI: 10.1016/j.str.2025.02.007
- Аннотация: Summary Nucleosomes are fundamental elements of chromatin organization that participate in compacting genomic DNA and serve as targets for the binding of numerous regulatory proteins. Currently, over 500 different nucleosome structures are known. Despite the large number of nucleosome structures, all of them were formed on only about twenty different DNA sequences. Using cryo-electron microscopy, we determined the structure of the nucleosome formed on a high-affinity Widom 603 DNA sequence at 4 Å resolution; an atomic model was built. We proposed an integrative modeling approach to study the nucleosomal DNA unwrapping based on the cryoelectron microscopy (cryo-EM) data. We also demonstrated the DNA unwrapping of the Widom 603 nucleosome using small angle X-ray scattering and single particle Förster resonance energy transfer measurements. Our results are consistent with the asymmetry of nucleosomal DNA unwrapping. Our data revealed the dependence of nucleosome structure and dynamics on the sequence of nucleosomal DNA.
- Добавил в систему: Армеев Григорий Алексеевич
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