Аннотация:There have been major advances in the development of replacement organs by tissue engineering (TE); however, one of the holy grails is still in the development of biomimetic structures that replicate the complex 3-D vasculature. Creation of bioartificial organs by decellularization shows greater promise in reaching the clinic compared to TE. However, current decellularization techniques require the use of chemical and biological agents, often in combination with physical force, which could result in damage to the matrix. Here we evaluate the use of boiling histotripsy (BH) to selectively decellularize large volumes of tissue. BH lesions (10–20 mm diameter) were produced in bovine liver with a clinical 1.2 MHz MR-HIFU system (Sonalleve, Philips, Finland), using thirty 10 ms pulses, and pulse repetition frequencies of 1–10 Hz. Peak acoustic powers corresponding to an estimated in situ shock front amplitude of 65 MPa were used. Macroscopic and histological evaluation revealed treatment conditions that produced decellularized lesions in which major fibrous structures such as stroma and vasculature remained intact while parenchymal cells were mostly lyzed. With further tailoring of the pulsing scheme parameters, this treatment modality could potentially be optimized for organ decellularization. [Work supported by NIH EB007643, K01-EB-015745-01, T32-DK007779, and NSBRI NASA-NCC 9-58.]