Transcriptome-wide analysis of protein synthesis: Ribosome profiling and beyond

Dmitriev, S.E.; Luppov, D.; Kats, L.M.; Anisimova, A.S.; Terenin, I.M.

Авторы: Dmitriev Sergey E., Luppov Daniil, Kats Leonid M., Anisimova Aleksandra S., Terenin Ilya M.
Сборник: Handbook of Translational Transcriptomics: Research, Protocols and Applications
Глава в коллективной монографии
Год издания: 2025
Издательство: Elsevier Inc.
Местоположение издательства: США
Первая страница: 231
Последняя страница: 298
Номер статьи: 11
DOI: 10.1016/b978-0-443-19110-7.00015-x
Аннотация: Ribosome profiling, or Ribo-Seq, has emerged as a powerful tool for deciphering protein synthesis and translational control, bridging our understanding of gene expression at the transcriptome and proteome levels. Through deep sequencing of ribosome-protected mRNA fragments, the approach allows for capturing a global snapshot of the cell translatome, encompassing both quantitative and positional information. Over its fifteen-year history, Ribo-Seq has been applied to cell cultures, animal tissues and organs, organelles, microbes, microbiota communities, and virus-infected cells across all kingdoms of life, uncovering the hidden coding potential of the human and many other genomes and revealing complex regulatory mechanisms underlying stress response, cancer, infectious diseases, development, and aging. The evolution of Ribo-Seq has led to the emergence of various derivative methods, including those for single-cell or tissue-specific analyses, focused on subcellular localization, selective ribosome-associated factors, or specific ribosome states, such as initiation complexes or collided ribosomes. This chapter reviews the recent advancements in research employing this revolutionary technique, providing examples of its utility in unraveling molecular mechanisms of protein synthesis, ribosome function, translation dynamics, quality control, human diseases, the effects of small molecule inhibitors, and identification of micropeptides. It also reviews major limitations, artifacts, and technical challenges that meet this technique and includes a detailed Ribo-Seq protocol for readers' reference.
Добавил в систему: Дмитриев Сергей Евгеньевич

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