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Rapid decline in MyHC I(β) mRNA expression in rat soleus during hindlimb unloading is associated with the AMPK dephosphorylationстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 января 2018 г.
- Авторы: Vilchinskaya NA, Mochalova EP, Nemirovskaya TL, Mirzoev TM, Turtikova OV, Shenkman BS
- Журнал: Journal of Physiology
- Том: 595
- Номер: 23
- Год издания: 2017
- Издательство: Blackwell Publishing Inc.
- Местоположение издательства: United Kingdom
- Первая страница: 7123
- Последняя страница: 7134
- DOI: 10.1113/JP275184
- Аннотация: One of the key events that occurs during skeletal muscle inactivation is a change in myosin phenotype, i.e. increased expression of fast isoforms and decreased expression of slow isoform of myosin heavy chain (MyHC). It is known that calcineurin/NFAT and AMP-activated protein kinase (AMPK) can regulate the expression of genes encoding MyHC slow isoform. Earlier, we found a significant decrease in phosphorylated AMPK in rat soleus after 24 h of hindlimb unloading (HU). We hypothesized that a decrease in AMPK phosphorylation and subsequent histone deacetylase (HDAC) nuclear translocation can be one of the triggering events leading to a reduced expression of slow MyHC. To test this hypothesis, Wistar rats were treated with AMPK activator (AICAR) for 6 d before HU as well as during 24-h HU. We discovered that AICAR treatment prevented a decrease in pre-mRNA and mRNA expression of MyHC I as well as MyHC IIa mRNA expression. 24-h HS resulted in HDAC4 accumulation in the nuclei of rat soleus but AICAR pretreatment prevented such an accumulation. The results of the study indicate that AMPK dephosphorylation after 24-h HU had a significant impact on the MyHC I and MyHC IIa mRNA expression in rat soleus. AMPK dephosphorylation also contributed to the HDAC4 translocation to the nuclei of soleus muscle fibres, suggesting an important role of HDAC4 as an epigenetic regulator in the process of myosin phenotype transformation. This article is protected by copyright. All rights reserved.
- Добавил в систему: Немировская Татьяна Леонидовна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | 2017-_J_Physiol.pdf | 2017-_J_Physiol.pdf | 916,0 КБ | 4 декабря 2017 [Nemirovskaya] |