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Complexes of Chlorin e6 with Pluronics and Polyvinylpyrrolidone: Structure and Photodynamic Activity in Cell Cultureстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 24 декабря 2014 г.
- Авторы: Zhiyentayev TM, Boltaev UT, Solov'eva AB, Aksenova NA, Glagolev NN, Chernjak AV, Melik-Nubarov NS
- Журнал: Photochemistry and Photobiology
- Том: 90
- Год издания: 2014
- Издательство: American Society for Photobiology
- Местоположение издательства: United States
- Первая страница: 171
- Последняя страница: 182
- Аннотация: Polymeric carriers are extensively used in photodynamic therapy (PDT) for increase of efficacy of photosensitizers. Here, we report the influence of nine Pluronic copolymers on phototoxicity of chlorin e6 (Ce6), in particular 5- to 7-fold rise in the phototoxicity caused by hydrophilic Pluronics F127, F108, F68 and F87 and practically no influence on Ce6 of more hydrophobic polymers. The revealed value of 0.2 mg mL-1 of Pluronic F127 concentration sufficient for half-of-maximal increase of Ce6 photodynamic activity proved to be close to 0.16 mg mL-1 inherent in well-documented carrier poly(N-vinylpyrrolidone) (PVP). The dissociation constants of Ce6 complexes with Pluronic F127 and PVP that were estimated from UV spectra were 0.252 and 0.036 mg mL-1 , respectively, indicating higher stability of Ce6 complex with PVP. According to the results of 1 H-NMR studies of Ce6 complexes, the porphyrin interacts not only with hydrophobic regions but also with hydrophilic sides of both polymers.
- Добавил в систему: Мелик-Нубаров Николай Сергеевич