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Outer membrane vesicles secreted by pathogenic and nonpathogenic Bacteroides fragilis represent different metabolic activitiesстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 19 октября 2017 г.
- Авторы: Zakharzhevskaya N., Vanyushkina A., Altukhov I., Shavarda A., Butenko I., Rakitina D., Nikitina A., Manolov A., Egorova A., Kulikov E., Vishnyakov I., Fisunov G., Govorun V.
- Журнал: Scientific reports
- Том: 7
- Год издания: 2017
- Издательство: Nature Publishing Group
- Местоположение издательства: United Kingdom
- Первая страница: 5008
- DOI: 10.1038/s41598-017-05264-6
- Аннотация: Numerous studies are devoted to the intestinal microbiota and intercellular communication maintaining homeostasis. In this regard, vesicles secreted by bacteria represent one of the most popular topics for research. For example, the outer membrane vesicles (OMVs) of Bacteroides fragilis play an important nutritional role with respect to other microorganisms and promote anti-inflammatory effects on immune cells. However, toxigenic B. fragilis (ETBF) contributes to bowel disease, even causing colon cancer. If nontoxigenic B. fragilis (NTBF) vesicles exert a beneficial effect on the intestine, it is likely that ETBF vesicles can be utilized for potential pathogenic implementation. To confirm this possibility, we performed comparative proteomic HPLC-MS/MS analysis of vesicles isolated from ETBF and NTBF. Furthermore, we performed, for the first time, HPLC-MS/MS and GS-MS comparative metabolomic analysis for the vesicles isolated from both strains with subsequent reconstruction of the vesicle metabolic pathways. We utilized fluxomic experiments to validate the reconstructed biochemical reaction activities and finally observed considerable difference in the vesicle proteome and metabolome profiles. Compared with NTBF OMVs, metabolic activity of ETBF OMVs provides their similarity to microreactors that are likely to be used for long-term persistence and implementing pathogenic potential in the host.
- Добавил в систему: Вишняков Иннокентий Евгеньевич