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Electroelution Into a Salt Trap: Reviving an Old‐School Approach to DNA Purificationстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 марта 2026 г.
- Авторы: Kalendar Ruslan, Ivanov Konstantin I., Samuilova Olga V., Burster Timo, Zamyatnin Andrey A.
- Журнал: Small Methods
- Год издания: 2026
- Издательство: John Wiley & Sons Inc.
- Местоположение издательства: United States
- Номер статьи: e02070
- DOI: 10.1002/smtd.202502070
- Аннотация: Recent advances in bioinstrumentation, such as the development of long-read sequencing, have reignited interest in methods for extracting long, intact nucleic acids from complex samples. One traditional method for this purpose is gel electrophoresis followed by electroelution from gel slices into a salt cushion. However, this method has become largely overlooked because its standard implementation is laborious, time-consuming, and incompatible with high-throughput workflows. In our recent work, we revisited this experimental approach and developed a simple, fast, and efficient method for purifying intact nucleic acids of varying lengths from complex samples. The method is available in both horizontal and vertical electrophoresis configurations, has the potential for automation and scalability, and is suitable for purifying high molecular weight (HMW) DNA for long-read sequencing. In this paper, we discuss the origins of the method, the stages of its development, and its advantages. The successful implementation of the method demonstrates how looking at a traditional technique from a new perspective can help meet the demands of next-generation technologies such as long-read sequencing. Our results highlight the importance of rethinking the applications of well-established yet underutilized methodological approaches in rapidly evolving fields such as biotechnology and bioinstrumentation.
- Добавил в систему: Замятнин Андрей Александрович
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