Mitigating Activity of Humic Substances under Water Deficiency Condition: Role of Phenolic Moietiesтезисы доклада

Работа с тезисами доклада

[1] Filippova O. I., Kulikova N. A., Perminova I. V. Mitigating activity of humic substances under water deficiency condition: Role of phenolic moieties // Book of Abstracts. Fourth International Conference of CIS IHSS on Humic Innovative Technologies From Molecular Analysis of Humic Substances – to Nature-like Technologies (HIT-2017). — ООО КЛУБ ПЕЧАТИ Москва, 2017. — P. 96–96. A comparative evaluation of the mitigating activity of humic substances (HS) derived from coal and peat towards the seedlings of wheat Triticum aestivum L. under water deficiency conditions has been performed. The studied HS included humic (HA), fulvic acids (FA) and hymatomelanic acid (HMA) isolated from coal and peat as they are the main sources for industrial humates designed for use in agriculture as plant growth regulators. All the studied HS were characterized by the methods of elemental analysis, size-exclusion chromatography and 13C NMR spectroscopy. Bioassay experiments was performed according to [1]. To create water deficiency, the hyperosmotic solution of polyethylene glycol with a molecular weight of 6,000 D (PEG 6000) with concentration 100 g/l (osmotic pressure –0.15 MPa) was used. Evaluation of the protective effect of HS was carried out at constant concentration of PEG- 6000 and concentrations of HS varying in the range 5–100 mg/L. Distilled water was used as a blank solution. Wheat seeds were placed in the Petri dishes containing solutions under study and left in the dark at 24C for 72 h. Then the length of roots and shoots of wheat seedlings was measured. Protective effect of HS was assessed based on comparison the lengths of roots and shoots of seedlings in the tested solutions to the blank one. Experiments demonstrated that HS were able to reduce partially the inhibition of wheat seedlings’ growth under water deficit caused by hyperosmotic solution of PEG- 6000. The protective effect of HS was revealed to enhance along with increasing the content of oxygen-substituted aromatic fragments in HS structure. Thus, the obtained results allow suggesting about increasing the protective effect of HS in relation to plants under conditions of water deficit by increasing the content of phenolic fragments. The observed mitigating activity of HS could not be explained by their binding with PEG-6000, which is a negatively charged polyelectrolyte. Therefore, HS possess the ability to reduce the negative effects of water deficiency due to their direct effect on plants. As mitigating activity of HS enhanced by increasing the content of phenolic fragments that may indicate the antioxidant activity of HS as the main mechanism of their protective activity under conditions of water deficit. However, to confirm this assumptions, an additional research aimed at the study of membranotropic properties of humic materials should be conducted. References 1. Klein O.I., Kulikova N.A., Konstantinov A.I., Fedorova T.V. et al. // Appl. Biochem. Microbiol. 2013. 49(3):287–295. This research was performed in the framework of state assignment (CITIS #116020110002-8).

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