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Application of the allogenic mesenchymal stem cells in the therapy of the bladder tuberculosisстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Yudintceva Natalia M., Bogolubova Irina O., Muraviov Alexandr N., Sheykhov Magomed G., Vinogradova Tatiana I., Sokolovich Evgenii G., Samusenko Igor A., Shevtsov Maxim A.
- Журнал: Journal of tissue engineering and regenerative medicine
- Год издания: 2017
- Издательство: John Wiley & Sons Ltd.
- Местоположение издательства: United Kingdom
- DOI: 10.1002/term.2583
- Аннотация: Urogenital tuberculosis (TB) often leads to contraction of the bladder, a reduction of the urinary reservoir capacity and, in the latest stage, to real microcystitis up to full obliteration. Bladder TB stage 4 is unsuitable for conservative therapy and cystectomy with subsequent enteroplasty is indicated. In the present study, using a model of bladder TB in New Zealand rabbits, the therapeutic efficacy of the interstitial injection of autologous bone-derived mesenchymal stem cells (MSCs) combined with standard anti-TB treatment in the restoration of the bladder function was demonstrated. For analysis of the MSC distribution in tissues, the latter were labelled with superparamagnetic iron oxide nanoparticles (SPIONs). In vitro studies demonstrated the high intracellular incorporation of nanoparticles and the absence of cytotoxicity on MSC viability and proliferation. A single dose administration of MSCs into the bladder mucosal layer significantly reduced the wall deformation and inflammation and hindered the development of fibrosis which was proven by the subsequent histological assay. Confocal microscopy studies of the bladder cryosections confirmed the presence of SPION-labelled MSCs in different bladder layers of the treated animals, thus indicating the role of stem cells in bladder regeneration.
- Добавил в систему: Юдинцева Наталия Михайловна