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Effects of short-term neonatal hyperthermia in Krushinsky-Molodkina audiogenic seizure prone rat strainтезисы доклада
- Авторы: Fedotova I.B., Surina N.M., Nikolaev G.M., Kostina Z.A., Poletaeva I.I.
- Сборник: 12th Goettingen Meeting of the German Neuroscience Society, March 22-25, 2017. Proceedings. Goettingen, 2017/
- Тезисы
- Год издания: 2017
- Место издания: NWG Goettingen
- Первая страница: T 13-9А
- Аннотация: Audiogenic epilepsy (AE) is the development of epileptiform seizures in response to a loud sound (which include wild run stages as well as stages of clonic and tonic convulsions). The physiological mechanisms of AE proneness were investigated with considerable details, while the biological basis of this pathology is less well understood. The selection of Russian audiogenic prone rat strain KM (Krushinsky-Molodkina) started at the end of 1940 and the strain was extensively used as the model of epilepsy and catatonic muscle state. Practically 100% of KM strain animals develop the AE seizure of highest intensity with very short latency. The remote effects of short (5 min) single hyperthermia (HPT) exposure (41-42 °С) of 14 days old KM rat pups will be presented. This data were obtained in 4 series of experiments (AE seizures, being tested at the age of 1 and 4 months). The heating of animals was performed using either the infrared lamp (series 1, 2 and 4), or (series 3) by placing animals in the thermostat (41 °С). The data were obtained for 85 rats in HPT group (48 males and 37 females) and for 96 control animals (42 males and 54 females). No significant differences between male and female groups in maximal AE seizure intensities were found at the ages of 1 and 4 months in both experimental and control groups and the respective data were pooled. At the age of 1 month part of animals was tested in the elevated plus maze (EPM) and their pain sensitivity thresholds (test tail flick) were determined. At the age of 1 month the animals from the HPT group developed significantly (p<0.01) less intense AE seizures than control rats, while the proportion of animals with most intense seizures was significantly lower in HPT group, and the proportion of rats with lack of seizures in response to sound was higher in treated animals (at the age of 1 month not all KM rats develop maximal AE fit). The postictal catalepsy, which is characteristic for KM rats, was significantly larger in HPT group. The pain sensitivity and elevated plus maze behaviour, tested at 1 month age, revealed significant differences from control group as well, which indicated the increased sensitization to pain in HPT animals together with increase in EPM test indices, which could be regarded as the plausible evidence of changes in anxiety. At the age of 4 months no significant differences between HPT and controls in AE proneness were found. The hyperthermia effects on seizures were previously analysed by other authors groups using status epilepticus model (subthreshold serial kainite or corazol injections), while the HPT effect on AE seizures after the single short term HPT was demonstrated in our study for the first time. The increase of body temperature as the stressor is accompanied by the increase of heat shock protein genes expression, which in turn could induce rather long-term physiological changes in CNS. The HPT used in this study could presumably induce changes in heat shock proteins expression, which (according to literature data) could be the cause of AE epileptiform seizures decrease. Supported by RFBR (grant № 15-04-01732) and the project NAAA-A16-116021660055-1. https://www.nwg-goettingen.de/2017/upload/file/Proceedings__NWG2017.pdf
- Добавил в систему: Лильп Ирина Германовна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | Nikolaev_2017_tezisyi_Goettingen.pdf | 119,9 КБ | 6 декабря 2017 [irinalilp] |