Reagentless Microsensor Based on Conducting Poly(3-aminophenylboronic Acid) for Rapid Detection of Microorganisms in Aerosolстатья
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Дата последнего поиска статьи во внешних источниках: 29 августа 2018 г.
Аннотация:We report on microsensor for direct detection of microorganisms in aerosol. Microsensor is based on interdigitated ultramicroelectrodes modified with electropolymerized 3-aminophenylboronic acid (3-APBA). Appearance of Penicillium chrysogenum in aerosol as well as further increase of its concentration lead to poly(3-APBA) conductivity increase found by impedance spectroscopy. According to Raman spectroscopy data, the presence of microorganism affects polymer conductivity similarly to binding of glucose to polymer which results in self-doping phenomenon. The elaborated detection technique is 100 times faster comparing to agar plate cultivation: it requires less than 20 minutes for analysis. Dynamic range of microsensor includes the upper limit for non-contaminated air in Russian hygienic standard (500 colony-forming units per cubic meter). The first reported reagentless detection of Penicillium chrysogenum in aerosol by conductivity increase reveals a prospect for creation of a promising alternative to conventional techniques for detection of microorganisms.