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Integrative approach for control of temperate bacteriophages in phage-based productsстатья
- Авторы: Rubalskii E.O., Aleshkin A.V., Afanasiev S.S., Aleshkin V.A., Galimzyanov Kh M., Umerova A.R., Rubalsky O.V., Ershova O.N., Rubalskaya E.E., Kiseleva I.A., Bochkareva S.S., Zul’karneev E.R., Akhmineeva A.Kh, Rubalsky M.O., Lunina I.O., Uskov V.V., Karnaukh M.M., Borisova O.Yu, Gadua N.T., Teplyi A.D., Rümke S., Salmoukas Ch, Kühn Ch, Haverich A.
- Журнал: Астраханский медицинский журнал
- Том: 12
- Номер: 3
- Год издания: 2017
- Первая страница: 56
- Последняя страница: 63
- Аннотация: Modern requirements for bacteriophages for therapeutic and preventive products recommend using only lytic (virulent) phages and avoiding temperate phages or other phage-like elements of genetic exchange. The presence of at least one temperate bacteriophage strain in the cocktail may lead to negative consequences, such as spread of antibiotic resistance, transfer of bacterial factors of pathogenicity, formation of bacteria resistant to phages. The sources of temperate phages in preparations may be both incorrectly characterized candidate strains of bacteriophages and lysogenic host bacteria cultures. Classical microbiological methods of temperate bacteriophages induction (heating, ultraviolet irradiation, mitomycin C) often give false negative results and therefore cannot provide a reliable approach of production control of phage-based preparations. As a basis of the developed methodology, we chose the sequencing of viral metagenomes including a database of known integrases.
- Добавил в систему: Ершова Ольга Николаевна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | elibrary_30693124_54245736.pdf | elibrary_30693124_54245736.pdf | 932,7 КБ | 26 июня 2018 [Pronkina_TE_nsi] |