Characteristics of regulation of the activity of glucokinase from rat liverстатья
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Дата последнего поиска статьи во внешних источниках: 19 сентября 2015 г.
Аннотация:A simple method for isolation of glucokinase from a soluble fraction of rat liver was proposed, making it possible to obtain the enzyme preparation characterized by high specific activity, native cooperative characteristics of the enzyme, and actually complete absence of other molecular forms of hexokinase. Dependence of the enzyme activity on the glucose concentration shows a pronounced sigmoidal behavior: with in the whole range of substrate concentrations at 25 and 2°C, the Hill coefficients are equal to 1.76 and 1.35, correspondingly, while the S0.5 values of glucose are 6.90 and 20 mM, respectively. The role of the kinetic reaction mechanism in the regulation of glucokinase activity in liver is under discussion.