A Moonlighting Human Protein Is Involved in Mitochondrial Import of tRNA

Baleva, M.; Gowher, A.; Kamenski, P.; Tarassov, I.; Entelis, N.; Masquida, B.

Авторы: Maria Baleva, Ali Gowher, Piotr Kamenski, Ivan Tarassov, Nina Entelis, Benoît Masquida
Журнал: International Journal of Molecular Sciences
Том: 16
Год издания: 2015
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 9354
Последняя страница: 9367
DOI: 10.3390/ijms16059354
Аннотация: In yeast Saccharomyces cerevisiae, ~3% of the lysine transfer RNA acceptor 1 (tRK1) pool is imported into mitochondria while the second isoacceptor, tRK2, fully remains in the cytosol. The mitochondrial function of tRK1 is suggested to boost mitochondrial translation under stress conditions. Strikingly, yeast tRK1 can also be imported into human mitochondria in vivo, and can thus be potentially used as a vector to address RNAs with therapeutic anti-replicative capacity into mitochondria of sick cells. Better understanding of the targeting mechanism in yeast and human is thus critical. Mitochondrial import of tRK1 in yeast proceeds first through a drastic conformational rearrangement of tRK1 induced by enolase 2, which carries this freight to the mitochondrial pre-lysyl-tRNA synthetase (preMSK). The latter may cross the mitochondrial membranes to reach the matrix where imported tRK1 could be used by the mitochondrial translation apparatus. This work focuses on the characterization of the complex that tRK1 forms with human enolases and their role on the interaction between tRK1 and human pre-lysyl-tRNA synthetase (preKARS2).
Добавил в систему: Каменский Петр Андреевич

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