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The reorganization of actin cytoskeleton and microtubulе system of human endothelial vein in the intercellular contacts formationстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 19 сентября 2015 г.
- Авторы: Shakhov A.S., Dugina V.B., Alieva I.B.
- Журнал: Cell and Tissue Biology
- Том: 9
- Номер: 4
- Год издания: 2015
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 299
- Последняя страница: 309
- DOI: 10.1134/S1990519X15040112
- Аннотация: Endothelial cells tightly joined to each other line the interior surface of all vessels in living organisms. They ensure the regulation of vascular permeability and exchange between the blood circulating in vessels and tissue fluids of those organs in which these vessels are located. In vitro the endothelial cell monolayer retains the basic barrier function of the native endothelium. We exploit endothelial cells growing in vitro as a model system to study the interaction between cytoskeleton and adhesion structures in endotheliocytes. In the current paper, cultured human vein endothelial cells were used to quantify cytoskeleton alterations from the cell spreading and formation of first cell-cell contacts to confluent monolayer formation. Actin filament system in the vein endothelial cells consists of cortical network and stress fibers arranged parallel to the substrate. The cells express two isoforms of non-muscle actin: beta- and gamma-cytoplasmic actins. Antibodies to beta-actin stain actin filament bundles whereas antibodies to gamma-actin visualize cortical and lamellar networks. Actin cytoskeleton organization was assessed by fluorescence intensity near the free edge and zone of cell-cell contacts. Fluorescence intensity of beta-actin structures was higher in the areas of cell-cell contacts. Fluorescence of gamma-actin was the highest at the leading edges of the lamellae and the weakest in the stable edges of the cells with formed cell-cell contacts. Formation of the endothelial monolayer was accompanied by changes in the microtubule system: the number of microtubules increased at the cell edge with the microtubules quantity in the area of already formed cell-cell contact always being higher than in leading edge region.
- Добавил в систему: Алиева Ирина Борисовна