Structure of the archaeal community in the Black Sea photic zoneстатья
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Дата последнего поиска статьи во внешних источниках: 26 августа 2016 г.
Аннотация:Qualitative and quantitative analysis of the structure of the archaeal community of the photic zone of the Black Sea water column was carried out. Real-time PCR revealed 2 × 104 archaeal cells/mL (4.2% of the total cell number) at a 15-m depth. The structure of archaeal communities in the subsurface water column was investigated using the sequencing by synthesis technology (Illumina/Solexa) of the 16S rRNA genes. The Marine Group II phylogenetic cluster belonging to the phylum Euryarchaeota was the most numerous
archaeal group (1.2–1.7 × 104 cells/mL). The Marine Group I phylogenetic cluster (phylum Thaumarchaeota) was the second most numerous group (40% of the free-living archaea or 7.7 × 103 cells/mL). Sequences of the 'Nitrosopumilus' cluster were revealed among Marine Group I sequences due to high homology (over 90%). A group of archaea belonging to the Deep-sea Hydrothermal Vent Euryarchaeotic Group 6 (DHVEG-6) (phylum Euryarchaeota) was also detected. The 16S rRNA gene sequences belonging to this cluster were revealed only in the suspension fraction. High homology level (over 90%) suggested classification of most DHVEG-6 sequences within the 'Parvarchaeum' cluster. In spite of a noticeable methane peak detected at 15-m depth, no sequences of methanogens were found.